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KMID : 0603820070130040349
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Journal of Experimental & Biomedical Science
2007 Volume.13 No. 4 p.349 ~ p.354
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Effects of Vaniltic Acid on the Cell Viability and Melanogenesis in Cultured Human Skin Melanoma Cells Damaged by ROS-Induced Cytotoxicity
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Ha Dae-Ho
Choi Yong-Ja
Yoo Seon-Mi
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Abstract
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The purpose of this study was to examine the effect of vanillic acid on the cell viability and melanogenesis in melanocytes damaged by reactive oxygen species (ROS). The human skin melanoma cells (SK-MEL-3) were cultured with various concentrations of hydrogen peroxide (H_2O_2). The cell viability for H_2O_2-induced cytotoxicity or vanillic acid against H_2O_2 was measured by XTT assay in these cultures. For the effect of vanillic acid on the melanogenesis, the tyrosinase inhibitory activity was measured by colorimetric assay at a wavelength of 490 nm, and melanin synthesis activity were assessed after cells were cultured in the media with or without various cencentrations of vanillic acid. In this study, H_2O_2 decreased cell viability dose- and time-dependent manners and XTT_{50} was determined at a concentration of 80 {mu}M, H_2O_2. Vanillic acid increased the cell viability dose dependently in human skin melanoma cells damaged by H_2O_2-induced cytotoxicity. In the tyrosinase inhibitory activity, vanillic acid supresssed tyrosinase activity in dosedependent manner, and also decreased significantly melanin synthesis activity compared with H_2O_2-treated group. From these results. It is suggested that H_2O_2-mediated cytotoxicity was highly by the toxic criteria of Borenfreund and Puerner and also, vanillic acid has the protective effect on ROS-induced cytotoxicity and melanogenesis in these cultures.
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KEYWORD
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Cytotoxicity, ROS, Melanogenesis, Vanilic acid
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